Isolation and functional charaterization of Regulatory T cells from Human peripheral blood

<p>Regulatory T cells are known to supress wide range of anti-tumour responses. It is now evident that Tregs are one among the main barriers for cancer immunotherapy. Until now there have been several methods described to isolate Tregs. It is also been documented that the immunosuppressive functions of CD4+CD25High Tregs in cancer patients is significantly higher than in healthy donors. This study has been designed to isolate CD4+CD25highCD127low/- Tregs from human PBMCs (healthy donors) and functionally characterize them based on their immunosuppression ability</p

Additional file 1: of A survey of ticks (Acari: Ixodidae) of companion animals in Australia

Questions answered by companion animal owners at veterinary clinics. (DOCX 55 kb

Additional file 3: of Endemic, exotic and novel apicomplexan parasites detected during a national study of ticks from companion animals in Australia

Table S3. Prevalence of Apicomplexa species Australia-wide and in each state and territory summarised for all tick species from all hosts, individual tick species from all hosts, and individual tick species from dogs, cats and horses. (XLSX 35 kb

Association of DACH1 protein with other breast cancer biomarkers.

<p>Association of DACH1 protein with other breast cancer biomarkers.</p

Kaplan-Meier plots modelling DACH1 expression with 5 year post-diagnostic a) specific survival, b) tumour recurrence, and c) distant metastasis.

<p>All were significant at p<0.001.</p

Association of DACH1 expression with clinicopathological factors. N = number of samples. Statistically significant p-values are in bold.

<p>Association of DACH1 expression with clinicopathological factors. N = number of samples. Statistically significant p-values are in bold.</p

Nuclear DACH1 immunostaining varied in intensity from being strong with expression in a high proportion of cells (a, b), to weak (c) or negative (d), in breast carcinoma.

<p>Nuclear DACH1 immunostaining varied in intensity from being strong with expression in a high proportion of cells (a, b), to weak (c) or negative (d), in breast carcinoma.</p

DACH1 Interactome.

<p>The association of DACH1 with the 100 best predictive genes in ER-positive tumours. The genes are represented as nodes and interactions as edges. The green edge is a positive interaction and the red edge is a negative interaction. The intensity of the interaction is represented in terms of the thickness of edge and the directionality with the arrow.</p